STED microscopy reveals crystal colour centres with nanometric resolution
نویسندگان
چکیده
منابع مشابه
STED microscopy reveals crystal colour centres with nanometric resolution
Because they have spin states that can be optically polarized and detected, fluorescent nitrogen vacancies in diamond1–3 have considerable potential for applications in quantum cryptography4,5 and computation6–8, as well as for nanoscale magnetic imaging9,10 and biolabelling11,12. However, their optical detection and control are hampered by the diffraction resolution barrier of far-field optics...
متن کاملResolution scaling in STED microscopy.
We undertake a comprehensive study of the inverse square root dependence of spatial resolution on the saturation factor in stimulated emission depletion (STED) microscopy and generalize it to account for various focal depletion patterns. We used an experimental platform featuring a high quality depletion pattern which results in operation close to the optimal optical performance. Its superior i...
متن کاملSub-Abbe resolution: from STED microscopy to STED lithography
Commonly, in stimulated emission depletion (STED) fluorescence nanoscopy, light of a wavelength located at the red tail of the emission spectrum of the dye is used to shrink the effective fluorophore excitation volume and thus to obtain images with sub diffraction resolution. Here, we demonstrate that continuous wave (CW) STED nanoscopy is feasible using STED wavelengths located at the emission...
متن کاملSTED microscopy: increased resolution for medical research?
Optical imaging is crucial for addressing fundamental problems in all areas of life science. With the use of confocal and two-photon fluorescence microscopy, complex dynamic structures and functions in a plethora of tissue and cell types have been visualized. However, the resolution of 'classical' optical imaging methods is poor due to the diffraction limit and does not allow resolution of the ...
متن کاملSTED super-resolution microscopy reveals an array of MINOS clusters along human mitochondria.
The mitochondrial inner membrane organizing system (MINOS) is a conserved large hetero-oligomeric protein complex in the mitochondrial inner membrane, crucial for the maintenance of cristae morphology. MINOS has been suggested to represent the core of an extended protein network that controls mitochondrial function and structure, and has been linked to several human diseases. The spatial arrang...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Nature Photonics
سال: 2009
ISSN: 1749-4885,1749-4893
DOI: 10.1038/nphoton.2009.2